ACUTE TOXICITY TEST (LETHAL DOSE50 ) OF ETHANOL EXTRACT OF FRUIT AND SEEDS OF BITTER GOURD (Momordica charantia Linn) ON MALE MICE Mus musculus

Zaini, Wawan S and Kurniati, Nining (2023) ACUTE TOXICITY TEST (LETHAL DOSE50 ) OF ETHANOL EXTRACT OF FRUIT AND SEEDS OF BITTER GOURD (Momordica charantia Linn) ON MALE MICE Mus musculus. In: Proceeding of The 1st Polkesraya International Conference. Poltekkes Kemenkes Palangka Raya, Palangka Raya, pp. 55-63. ISBN 2987-8659

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Abstract

The purpose of this study was to show that bitter melon fruit and seeds were safe for consumption by testing the toxicity by determining the LD 50 value of the ethanol extract of bitter melon fruit and seeds using the Thompson-Weil method and their effect on animal behavior. The test animals used were 23 male white mice (Mus musculus) with an average weight of 25 grams and divided into 5 groups.Giving a solution of bitter melon fruit and seed extract orally with various doses: 100 mg/kgbw, 50 mg/kgbw, 25 mg/kgbw and 12.5 mg/kgbw and administration of sterile distilled water as a control group. Mice were observed individually for 1 hour, 2 hours, 4 hours and 24 hours after administration of the extract by looking at the number of dead animals and the visible toxic symptoms. After that, it was observed for 2-14 days after treatment. The results of the research on bitter melon extract found that 1 mice died at a dose of 100 mg/kgbw (20%), while at other doses there were no mice that died. The results of the research on bitter melon seed extract were 1 mice died at a dose of 100 mg/kgbw (20%), at a dose of 50 mg/kgbw, 2 mice died (40%), and at a dose of 12.5 mg/kgbw, mice died much 1 mice (20%), while at other doses no mice died. This indicates that the extract of bitter melon fruit and seeds is included in the non-toxic category.The administration of the extract test material did not cause toxic symptoms in which all rats had normal activities. So that bitter melon and bitter melon seeds are safe for consumption.

Key words : Momordica charantia,_ Extract, Toxicity_ Mus musculus, ThompsonWeil

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